Liquid process chromatography with LEWA systems

Purifying (downstream processing) materials using chromatography is mandatory for manufacturing fine chemicals.

Whether diaphragm metering pumps or gradient mixers, at LEWA, we configure products according to your requirements. Our systems are appealing due to their reproducible metering accuracy, large adjustment ranges and high product quality that have been tried-and-tested in an industrial production scale.

Your individual solution for downstream processing

LEWA supplies diaphragm metering pumps and complete metering systems for various processes, e.g. gradient mixers for gradient generation or customer-specific chromatography systems, which are designed precisely to customer specifications.

Advantages:

  • Precise flow rates (gradually or linearly variable., 1-99%)
  • Precisely reproducible positioning accuracy (0.5%)
  • Hermetically tight
  • Very wide, reproducible dynamic range
  • High availability, reliability, and process assurance
  • Reliable validation due to their high precision
  • Inline cleanable and steamable (CIP/SIP), optional venting
  • Hygienic pumps available (fluid-wetted electro-polished, ASME-BPE, EHEDG-certified)
  • Design available for clean room installation
  • Complete skid design with all process relevant attachments for integration in chromatography systems possible
  • Comprehensive documentation of qualification and certification
  • IQ/OQ support
  • Explosion protection at customer request

Areas of application: LEWA metering pumps, metering systems and gradient mixers are used in downstream processing, including in the following areas:

Low and medium-pressure chromatography, for example

  • Preparative liquid column chromatography
  • Normal and reversed phase chromatography
  • Ion exchanger chromatography
  • Affinity chromatography, etc.
  • Downstream processing for purifying vaccines, innovative therapeutic proteins, monoclonal antibodies, hormones and blood plasma products

High pressure chromatography

  • High-pressure liquid process chromatography (HPLC)
  • Preparative countercurrent chromatography with supercritical CO2 using SFC (supercritical fluid chromatography)

Get in touch today so that we can talk about a strategy for implementing your downstream processing requirements!

Background: Process description of downstream processing

Chromatography offers a broad spectrum of physical methods for separation and/or analysis of complex mixtures. The components to be separated are divided into two phases: The stationary phase in one column and the mobile phase, which flows through the stationary packing (fluid or gas).

How does chromatography work?

A mixture of different components enters into a liquid or gas process chromatography process. The different separating and chromatography solutions are pumped through the system at different speeds. The separation of the substance mixture is done through interactions of differing strength between the stationary and mobile phases in the system. The smaller the affinity of a molecule for the stationary phase, the shorter the time in a chromatography column and vice versa.

Why is chromatography so special and important?

In the chemical and bioprocessing industry, there is a need to separate a product from a complex mixture and to rid it of contamination. This is one important step in the entire chain of processing (down streaming/cleaning), to produce the product in the needed purity.

Currently there is a wide selection of methods with which the industry can reach these goals. Chromatography is a very special and selective method of separation for numerous reasons, which has been used especially in the production area since decades.

First of all, complex mixtures can be separated with high precision; also very complex molecules, such as proteins, can be gently separated with chromatography, for example. Basically, any soluble or volatile substance can be cleaned, if the correct adsorbent agents and carrier liquid/mobile phase are used under selected and controlled process parameters (temperature, pH value, flow speed, conductivity, etc.)

Chromatographic processes are perfect for the separation or cleanup of sensitive products, espescially established in the pharma sector.

Products

LEWA ecoflow diaphragm metering pump

LEWA ecoflow diaphragm metering pump

The ecoflow® diaphragm metering pump from LEWA is a true all-around talent in the field of metering and mixing and can be used in all industries.

LEWA ecoflow sanitary/hygienic

LEWA ecoflow sanitary food pump

The ecoflow® diaphragm metering pump in sanitary/hygienic design is the ideal pump for food and pharmaceutical products.

LEWA ecodos sanitary/hygienic

LEWA ecodos food pump

The LEWA ecodos® diaphragm metering pump with its sanitary/hygienic design is the ideal pump for food and pharmaceutical products in the low-pressure range.

LEWA intellidrive diaphragm metering pumps

LEWA intellidrive diaphragm metering pump

Use servomotor-controlled diaphragm metering pumps for precision metering and mixing of fluids in metering plants and systems.

LEWA FC laboratory pump

LEWA micrometering pump FC

The smallest flow rates and precise metering make LEWA micro-metering pumps of the FC series the first choice for laboratory pumps.

LEWA ecoflow process diaphragm pump

LEWA high pressure pump for process engineering

The LEWA ecoflow process diaphragm pump is the top performer among innovative and universal process pumps for high-pressure process technology.

LEWA EcoPrime Chromatography

LEWA EcoPrime Chromatography System

For application-specific chromatography in the chemical, pharmaceutical, and biotechnology industries: LEWA EcoPrime® supports your processes. Contact us!

Customized production units

LEWA metering and mixing system

LEWA develops complete systems for metering, mixing, and filling fluids. Contact us and let us advise you!

Customer-specific metering systems

LEWA metering system

LEWA provides you with custom-designed metering systems according to your specifications. Put your trust in the experience gained from 60 years of metering technology in various industries.

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